Graphic User Interface FAQ — Can I have Tk events handled while waiting for I/O?
On platforms other than Windows, yes, and you don't even need threads!
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On platforms other than Windows, yes, and you don't even need threads! But you'll have to restructure your I/O code a bit. Tk has the equivalent of Xt's !XtAddInput call, which allows you to register a callback function which will be called from the Tk mainloop when I/O is possible on a file descriptor. See tkinter-file-handlers.
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Python Documentation — Doc/faq/gui.rst :: Can I have Tk events handled while waiting for I/O? ↗Revision 948fd7e5c084 · PSF-2.0